3D-cultured hADSCs-derived exosomes deliver circ_0011129 to synergistically attenuate skin photoaging.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Skin photoaging is primarily induced by ultraviolet (UV) exposure, involving mechanisms such as reactive oxygen species (ROS) accumulation, matrix metalloproteinase (MMP)-mediated collagen degradation, and cathepsin (e.g., Cathepsin K)-driven elastin denaturation and aggregation. Although circular RNA (circRNA) shows significant potential in regulating skin photoaging, its clinical translation remains challenging due to poor in vivo stability and targeted delivery efficiency. This study aimed to construct a 3D-cultured human adipose-derived mesenchymal stem cell (hADSC)-derived exosome (3D-Exo) loaded with circ_0011129 (3D-circ-Exo) and investigate its protective effects and molecular mechanisms against chronic UV-induced damage in human dermal fibroblasts (HDFs). A circ_0011129-overexpressing hADSC cell line was established via lentiviral transfection. Exosomes were isolated, and circRNA integrity was validated through divergent/convergent primer amplification, sequencing, and RNase R digestion. A chronic photoaging HDFs model was induced by 7-day UVA irradiation (5 J/cm 2 /d). Cellular senescence (SA-β-gal staining, p53/p21/p16 expression) and extracellular matrix degradation (collagen I, elastin) were assessed. Therapeutic effects were evaluated across four groups: light-shielded control, UVA-irradiated control, 3D-Exo + UV, and 3D-circ-Exo + UV. The 3D-circ-Exo carrier succ

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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