3D endothelial network formation in hydrogels improved by stromal cells and specific growth factors.
Acimovic I., Chochola V., Herrera JL., Hampl A., Jaros J.
Laboratory Study, published in Sci Rep (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Sci Rep (2025)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 41285921
- PMCID
- PMC12644823
- DOI
- 10.1038/s41598-025-25381-x
- Citations
- 2
Abstract (original English)
The limiting factor in the current state-of-the-art bioengineering of human tissues/organoids in vitro is the lack of functional vasculature that would support growth, metabolic waste removal, and oxygen supply of the formed constructs. The three-dimensional (3D) tissues can be formed by utilization of specific cell types, different hydrogels and scaffolds that provide a physical support, in addition to specific medium composition. Establishment of chemically defined xeno-free conditions is of great interest when keeping in mind the transition from basic research to therapeutic applications. In this study, we present the formation of vascular network of human umbilical vein endothelial cells co-cultured with stromal cells (SCs), human dental pulp SCs or human adipose SCs, in natural hydrogels (Matrigel, GelMA) as well as xeno-free VitroGel hydrogels. As medium composition plays a pivotal role in the successful generation of biological constructs in vitro, we evaluated the potency of specific growth factors (GFs) to induce vascular network formation in 3D hydrogels. We confirmed that vascular endothelial growth factor (VEGF), the most used pro-angiogenic factor, is not mandatory in culture medium for vascular network formation. Rather the endothelial cell (EC) network in co-culture of ECs and SCs can be formed through stimulation of SCs with GFs such as insulin-like growth facto
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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