456 Pluripotent stem cell differentiation to osteogenic and chondrogenic precursor cells
Stice S.
Laboratory Study, published in J Anim Sci (2018) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- J Anim Sci (2018)
- Reported sample size
- —
- Source database
- Europe PMC
- PMCID
- PMC6285712
Abstract (original English)
Abstract Osteo/chondrogenic progenitor (OCP) cells were derived from pluripotent stem cells (PSC) including embryonic and induced pluripotent stem cells. The PSC were induced to undergo an epithelial to mesenchymal transition resulting in OCP cells that had unique developmental characteristics. After transitioning to a mesenchymal phenotype, the OCP were highly proliferate as an adherent cell monolayer and maintained karyotype stability for more than 75 population doublings. OCP cells readily differentiated in vitro into osteogenic and chondrogenic subtypes. In contrast, tissue sourced adult mesenchymal stem cells (MSC) have broad potential but present potential drawbacks, including: 1) variability in starting population; 2) limited lifespan; 3) heterogeneous multilineage differentiation potential. These issues are overcome by PSC derived OCP cells which do not require manual selection or sorting to produce a unique cell type. However, the OCP cells unexpectedly lacked adipogenesis potential following exposure to adipogenic differentiation culture conditions. The developmental mechanism(s) responsible for OCP cell adipogenesis deficiency is largely unknown. These characteristics may be related to their ontologically naïve state of development compared to adult sourced MSC. We showed that OCP cells are epigenetically distinct from adult MSC given promoters for genes related to a
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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