459 Late-Breaking: Thyroid-stimulating hormone increases mitochondrial oxidative capacity in an in vitro study of equine skeletal muscle
Latham C., Welsh T., Fluckey J., Smith S., White S.
Laboratory Study with a reported sample of 3, published in J Anim Sci (2018) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
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- Study type
- Laboratory Study
- Journal
- J Anim Sci (2018)
- Reported sample size
- 3
- Source database
- Europe PMC
- PMCID
- PMC6285726
Abstract (original English)
Abstract Thyroid-stimulating hormone (TSH) has been shown to increase mitochondrial number and function in human epidermis (Poeggeler et al., FASEB J. 24:1525–1531; 2010). Extrathyroidal action of TSH has not been examined in horses. Therefore, we aimed to test the hypothesis that TSH would increase mitochondrial density and activity in muscle from sedentary mature (n=3; 11 ± 4.6 yr) and aged (n=4; 19 ± 3.6 yr) Quarter horses (4 mares, 3 geldings). Triceps brachii muscle samples (600 mg) were placed into chilled PBS for immediate transport to the laboratory. Tissue from each horse was separated into 8 wells (75 mg/well) containing 2 mL incubation media (DMEM, 5% fetal bovine serum, 10% horse serum, 50 ug/mL gentamycin) with 2 replicates of 4 doses of bovine pituitary TSH (Millipore Sigma): 0 (CON), 2.5 (T2.5), 10 (T10) or 20 (T20) mIU TSH/mL. Alamar Blue (10%) was added to one replicate for each TSH concentration to assess metabolic rate; the second replicate was used for analysis of oxidative capacity (PCI+II) via high-resolution respirometry and mitochondrial number (citrate synthase activity; CS) and function (cytochrome c oxidase; CCO) by colorimetry. Samples were incubated for 18 h at 37oC, 5% CO2. For each horse, values were normalized to CON and data were analyzed using PROC MIXED (SAS v9.4) with TSH dose, age, sex and all interactions as fixed effects. Citrate synthase
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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