AB110. In vitro evaluation of endothelial progenitor cells from adipose tissue as potential angiogenic cell sources for bladder angiogenesis
Laboratory Study, published in Transl Androl Urol (2016) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Transl Androl Urol (2016)
- Reported sample size
- —
- Source database
- Europe PMC
- PMCID
- PMC4842621
Abstract (original English)
Objective Autologous endothelial progenitor cells (EPCs) might be alternative angiogenic cell sources for vascularization of tissue-engineered bladder, while isolation and culture of EPCs from peripheral blood in adult are usually time-consuming and highly inefficient. Recent evidence has shown that EPCs also exist in the adipose tissue. As adipose tissue is plentiful in the human body and can be easily harvested through a minimally invasive method, the aim of this study was to culture and characterize endothelial progenitor cells from adipose tissue (ADEPCs) and investigate their potential for the neovascularization of tissue-engineered bladder. Methods Adipose stromal vascular fraction (SVF) was isolated and used for the culture of ADEPCs and adipose derived stem cells (ADSCs). Rat bladder smooth muscle cells (RBSMCs) were isolated and cultured from rat bladder. Cells were characterized by immunofluorescence staining and flow cytometric analysis. The capabilities of cell proliferation, DiI-Ac-LDL uptake, and UEA-1 binding were evaluated. Real-time RT-PCR and ELISA were performed to assess the expression of angiogenic factors. ADEPCs were seeded onto matrigel and bladder acellular matrix (BAM), and the capability of tube formation in these scaffolds was evaluated. Results After SVF was cultured for one week, ADEPCs with typical cobblestone morphology emerged and could be isola
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
How we grade evidenceBrowse all related research
Filter the research library by this study's title keywords, author, or publication year.