AB187. Establishment of the high resolution melting analysis with unlabeled probes for genotyping CCL2-2518 T > C
Laboratory Study, published in Transl Androl Urol (2014) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Transl Androl Urol (2014)
- Reported sample size
- —
- Source database
- Europe PMC
- PMCID
- PMC4708414
Abstract (original English)
Objective To establish a high resolution melting analysis with unlabeled probes for genotyping CCL2-2518 T > C. Methods Two unlabeled probes were designed. One is complementary to wild type and another is complementary to the mutant type. A total of 71 health people were performed by HRM with unlabeled probes for genotyping CCL2-2518 T > C, and TaqMan method was used to verify the results from above. Results The results using the HRM with unlabeled probes were consistent with that using TaqMan method. The difference between the temperatures in two probe peaks by using the probe complementary to wild type is 2.1°C; and the difference between the temperatures in two probe peaks by using the probe complementary to mutant type is 5.5°C. Conclusions Establishment the high resolution melting analysis with unlabeled probes for genotyping CCL2-2518 T > C is a simple, economic, sensitive, accurate, and fast technique, which could be used in clinical inspection.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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