Aberrantly expressed long noncoding RNAs in adipose-derived mesenchymal stem cells differentiation to nucleus pulposus-like cells.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Stem cell-based therapy holds promise for intervertebral disc degeneration (IDD) regeneration, but the underlying mechanisms require further elucidation. Long noncoding RNAs (lncRNAs) have been implicated in physiological processes such as apoptosis and differentiation. While numerous studies have investigated messenger RNAs (mRNAs) and lncRNAs in normal versus degenerated nucleus pulposus (NP), the differential expression of lncRNAs and mRNAs during stem cell differentiation into NP-like cells remains poorly characterized. This study aimed to identify differentially expressed mRNAs and lncRNAs during the differentiation of human adipose-derived mesenchymal stem cells (hADSCs) into NP-like cells and to explore the associated signaling pathways and regulatory networks. hADSCs were induced to differentiate into NP-like cells using a cytokine cocktail. Differentiation was confirmed by assessing marker gene expression via PCR and immunofluorescence staining. RNA sequencing (RNA-seq) was employed to profile lncRNA and mRNA expression during differentiation. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were used to identify the biological functions and pathways associated with the significantly altered genes. 500 lncRNAs (217 up and 283 down) and 601 mRNAs (269 up and 332 down) were significantly differentially expressed during hADSC differen

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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