Accelerating effect of Shilajit on osteogenic property of adipose-derived mesenchymal stem cells (ASCs).
Kangari P., Roshangar L., Iraji A., Talaei-Khozani T., Razmkhah M.
Laboratory Study, published in J Orthop Surg Res (2022) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- J Orthop Surg Res (2022)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 36153551
- PMCID
- PMC9509599
- DOI
- 10.1186/s13018-022-03305-z
- Citations
- 9
Abstract (original English)
Background: Shilajit, as a herbomineral natural substance, has been most widely used remedy for treating a numerous of illness such as bone defects in Iran traditional folk medicine since hundreds of years ago. The aim of the present study was to explore the effect of Shilajit on the osteogenic differentiation of human adipose-derived mesenchymal stem cells (ASCs) in two- and three-dimensional cultures. Materials and methods ASCs were isolated and seeded in three-dimensional (3D) 1% alginate (Alg) hydrogel with or without Shilajit at a density of 3 × 10 5 cell/mL. For two-dimensional (2D) cultures, 3 × 10 4 ASCs /mL were seeded into culture plates and treated with 500µg/mL Shilajit. Then, characterization was done using electron microscopy (SEM)/energy dispersive X-ray spectroscopy (EDX), alkaline phosphatase (ALP) activity, Alizarin red staining, and Raman confocal microscopy. Results Adding Shilajit had no impact on the Alg scaffold degradability. In the 3D Alg hydrogel and in the present of osteogenic medium (OM), Shilajit acted as enhancer to increase ALP activity, also showed osteoinductive property in the absence of OM compared to the 2D matched groups at all time points (days 7 and 21 both P < 0.001, for 14 days P < 0.001 and P < 0.05, respectively). In addition, calcium deposition was significantly increased in the cultures exposed to Shilajit compared to 2D matched gro
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
How we grade evidenceBrowse all related research
Filter the research library by this study's title keywords, author, or publication year.