Activation of Cx43 Hemichannels Induces the Generation of Ca 2+ Oscillations in White Adipocytes and Stimulates Lipolysis
Turovsky EA., Varlamova EG., Varlamova EG., Turovskaya MV.
Animal Study, published in Int J Mol Sci (2021) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Int J Mol Sci (2021)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 34360859
- PMCID
- PMC8347185
- DOI
- 10.3390/ijms22158095
- Citations
- 21
Abstract (original English)
The aim of the study was to investigate the mechanisms of Ca 2+ oscillation generation upon activation of connexin-43 and regulation of the lipolysis/lipogenesis balance in white adipocytes through vesicular ATP release. With fluorescence microscopy it was revealed that a decrease in the concentration of extracellular calcium ([Ca 2+ ] ex ) results in two types of Ca 2+ responses in white adipocytes: Ca 2+ oscillations and transient Ca 2+ signals. It was found that activation of the connexin half-channels is involved in the generation of Ca 2+ oscillations, since the blockers of the connexin hemichannels-carbenoxolone, octanol, proadifen and Gap26-as well as Cx43 gene knockdown led to complete suppression of these signals. The activation of Cx43 in response to the reduction of [Ca 2+ ] ex was confirmed by TIRF microscopy. It was shown that in response to the activation of Cx43, ATP-containing vesicles were released from the adipocytes. This process was suppressed by knockdown of the Cx43 gene and by bafilomycin A1, an inhibitor of vacuolar ATPase. At the level of intracellular signaling, the generation of Ca 2+ oscillations in white adipocytes in response to a decrease in [Ca 2+ ] ex occurred due to the mobilization of the Ca 2+ ions from the thapsigargin-sensitive Ca 2+ pool of IP 3 R as a result of activation of the purinergic P2Y1 receptors and phosphoinositide signaling pat
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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