Level D· Scientific groundwork from lab and animal studiesLaboratory StudyPubMed

Adipogenic Differentiation of Human Adipose-Derived Mesenchymal Stromal/Stem Cells (ASCs).

Adam LT., Pitere RR., Ambele MA., Pepper MS.

Laboratory Study, published in Methods Mol Biol (2025) — summary generated from the PubMed abstract.

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Level D· Scientific groundwork from lab and animal studiesEvidence level of this study

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

  • Level A · Stronger Clinical Evidence
  • Level B · Emerging clinical evidence with positive signals
  • Level C · Early human research exploring benefits
  • Level D · Scientific groundwork from lab and animal studies
  • Emerging · Emerging topic under active research
Read the A–D evidence level guide

This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.

Study type
Laboratory Study
Journal
Methods Mol Biol (2025)
Country
United States
Reported sample size
—
Source database
PubMed
PMID
40445280
DOI
10.1007/978-1-0716-4607-6_7

Abstract (original English)

Adipose-derived stromal/stem cells (ASCs) from human fat tissue samples offer a valuable model to study adipogenesis in vitro. A hormonal cocktail that promotes growth and differentiation can be used in experimental procedures to facilitate the conversion of ASCs into adipocytes. This protocol describes methods for adipogenic differentiation of primary human ASCs in vitro, outlining flow cytometry quantification and fluorescence microscopy qualitative assessment of the adipogenic process.

What this study does not prove

  • • This study does not prove SVF is an approved treatment or a replacement for standard care.
  • • This is preclinical work; animal or laboratory results cannot be applied to humans.

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

How we grade evidence
HumansMesenchymal Stem CellsAdipogenesisAdipose TissueFlow CytometryCell DifferentiationCells, CulturedAdipocytesCell Culture TechniquesMicroscopy, Fluorescence

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