Adipogenic Differentiation from Human Embryonic Stem Cells Through Formation of Embryoid Bodies.
Olmedo-Suárez MA., Sánchez-Ramírez E., Aguilar-Arnal L.
Laboratory Study, published in Methods Mol Biol (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Methods Mol Biol (2025)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 40445274
- DOI
- 10.1007/978-1-0716-4607-6_1
Abstract (original English)
In the context of adipogenesis, new adipocytes arise from the differentiation of pluripotent stem cells, including embryonic stem cells (hESCs). This process involves two fundamental phases: lineage commitment and terminal differentiation. This chapter elucidates a comprehensive 22-day protocol designed for the differentiation of the hESC cell line H9 toward the adipogenic lineage.During the initial phase, we initiate the differentiation process by forming embryoid bodies (EBs). This is achieved by culturing hESCs in an ultralow attachment plate for a duration of 12 days, during which the EBs are exposed to retinoic acid (RA) for a period of 3 days. Subsequently, adipocyte induction is facilitated by transferring EBs onto Geltrex-coated 6-well plates and subjecting them to adipogenic differentiation medium for an additional 10 days. To assess the successful internalization of lipids within the adipocytes, we employ Oil Red O staining. This protocol provides a robust framework for studying adipogenesis from pluripotent stem cells, offering insights into the intricate process of adipocyte development and serving as a valuable tool for related research endeavors.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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