Adipose-derived mesenchymal stem cell-derived exosomes alleviate overwhelming systemic inflammatory reaction and organ damage and improve outcome in rat sepsis syndrome.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

This study tested the hypothesis that healthy adipose-derived mesenchymal stem cell (ADMSC)-derived exosomes (HMSC EXO ) and apoptotic (A) (induced by 12 h hypoxia/12 h starvation)-ADMSC-derived exosomes (AMSC EXO ) were comparably effective at alleviating sepsis syndrome [SS; induced by cecal-ligation and puncture (CLP)]-induced systemic inflammation and reduced organ damage and unfavorable outcomes in rats. SD rats were divided into sham control (SC), SS only, SS + HMSC EXO (100 µg intravenous administration 3 h after CLP), and AMSC EXO . By day 5 after CLP procedure, the mortality rate was significantly higher in SS than in SC and HMSC EXO (all P < 0.01), but it showed no significant different between SC and HMSC EXO , between AMSC EXO and HMSC EXO or between SS and AMSC EXO (P > 0.05). The levels of inflammatory mediators in circulation (CD11 b/c /Ly6G/MIF), bronchioalveolar lavage (CD11 b/c /Ly6G) and abdominal ascites (CD11 b/c /CD14/Ly6G/MIF) were highest in SS, lowest in SC and significantly higher in AMSC EXO than in HMSC EXO (all P < 0.001). The circulating/splenic levels of immune cells (CD34+/CD4+/CD3+/CD8+) were expressed in an identical pattern whereas the T-reg+ cells exhibited an opposite pattern of inflammation among the groups (all P < 0.001). The protein expressions of inflammation (MMP-9/MIF/TNF-α/NF-κB/IL-1β) and oxidative stress (NOX-1/NOX-2/oxidized prote

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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