Adipose-derived stem cells alleviate acute pancreatitis by inhibiting ferroptosis and oxidative damage in canines.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Abstract Background Canine acute pancreatitis (AP) is a common exocrine pancreatitis disease that can lead to systemic inflammatory response syndrome and multi-organ failure. This study aims to investigated the potential therapeutic benefits of adipose-derived stem cells (ADSCs) and conditioned medium (CM) in managing canine AP and the role in ferroptosis regulation. Methods Sixteen dogs were randomly divided into control (CON), AP, ADSCs and CM group. The AP model were established by injecting sodium taurocholate (5%, 0.1 mL/kg) and trypsin (3500 U/kg) through the pancreaticobiliary duct. ADSCs (1×10 6 /kg) and CM (0.1 mL/kg) were injected intravenously at 6 h after surgery, and the roles on ferroptosis and oxidative stress were analyzed. In addition, the changing pattern of ferroptosis and oxidative stress were investigated by LPS-induced cellular inflammation model of AR42J in vitro. Results Our study showed that ferroptosis occurs in the pancreas during AP, as evidenced by significant iron accumulation, with suppressed glutathione peroxidase 4 (GPx4) expression and increased transferrin receptor-1 (TFR1) and ferritin heavy chain (FTH). ADSCs and ADSCs-CM treatment achieved pathological remission and effectively restored abnormal amylase (AMY), lipase (LIPA) levels. ADSCs-CM showed similar ferroptosis alleviating effects as ADSCs treatment, with reduced iron accumulation and

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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