Adipose-derived stem cells-conditioned medium (ADMSCs-CM) improves testicular function and sperm DNA damage via Nrf2 antioxidant signaling pathways in rat unilateral varicocele.
Khosravi-Farsani S., Khadivi F., Rafiei M., Ahmadi R., Khosravi-Farsani R., Amini-Khoei H.
Laboratory Study, published in Stem Cell Res Ther (2026) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Stem Cell Res Ther (2026)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 42393810
- DOI
- 10.1186/s13287-026-05145-8
Abstract (original English)
Varicocele is an excessive dilation of the pampiniform plexus. Although the accurate mechanism of varicocele-induced infertility is still unknown, oxidative stress plays a major function. A great attention has recently been attracted towards the MSCs' secreted factors for the treatment of diseases. So, we investigated the effect of adipose derived stem cells-conditioned medium (ADMSCs- CM) on Nrf2 signaling pathway, downstream antioxidant enzymes, sperm DNA and acrosome damage following varicocele surgery. Rats were divided into sham, varicocele, Varicocele + DMEM/F-12, and ADMSCs-CM groups. ADMSCs were isolated, confirmed, and ADMSCs-CM was prepared. Spermatogenesis quantification was done by H& E-stained testicular sections. The percentage of sperm DNA fragmentation and acrosome integrity was assessed through acridine orange and coomassie blue stainings. The testicular levels of MDA, TAC, SOD, and CAT were assessed. Relative expression of Nrf2/ Ho-1 genes evaluated through Real-time PCR. Mean percentage of sperm acrosome integrity, TDI, SPI, and Johnsen's criteria decreased (p < 0.0001), mean percentage of sperm DNA fragmentation (p < 0.0001), and testicular MDA (p < 0.001) significantly increased in the varicocele group. Unilateral varicocele reduced notably testicular levels of TAC (p < 0.0001), SOD (p < 0.01), and CAT (p < 0.05). ADMSCs-CM improved significantly sperm acro
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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