Adipose-Derived Stem Cells From Human Lumbar Subcutaneous Tissue in Obesity: Inflammatory Microenvironment Promotes Differentiation Capacity.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Background Adipose-derived stem cells (ADSCs), possessing multipotent differentiation potential, hold considerable promise for clinical stem cell therapy applications. ADSCs isolated from obese individuals may exhibit differences compared to those derived from individuals of normal weight. Methods Adipose tissues were collected from donors with body mass index (BMI) values within the standard range (Group N) and the obese range (Group O), respectively. ADSCs were isolated from these tissues. The proliferative capacity, phenotypic characteristics, and functional properties of the derived ADSCs were comparatively assessed. To mimic the inflammatory microenvironment of adipose tissue in obese individuals in vitro, ADSCs from Group N were prestimulated with inflammatory cytokines and designated as Group Pre, followed by assessment of their phenotypic characteristics and functional properties. Proteomic analysis was performed on the conditioned medium collected from the ADSCs cultures. Results ADSCs of Group N exhibited stronger proliferation capacity than Group O. However, Group N demonstrated lower adipogenic and osteogenic differentiation potential compared to Group O, along with reduced expression of stemness-associated surface markers and genes. Compared to Group N, Group Pre showed no significant change in proliferative capacity; however, it exhibited enhanced adipogenic and o

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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