Adipose-derived stem cells promote human dermal fibroblast function and increase senescence-associated β‑galactosidase mRNA expression through paracrine effects.
Shen X., Du Y., Shen W., Xue B., Zhao Y.
Laboratory Study, published in Mol Med Rep (2014) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Mol Med Rep (2014)
- Country
- Greece
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 25310816
- DOI
- 10.3892/mmr.2014.2627
- Citations
- 10
Abstract (original English)
Adipose‑derived stem cells (ADSCs) are known to secrete various cytokines, which affect fibroblast function through paracrine effects. In the present study, the paracrine effects of ADSCs on the function and senescence of young and aged human dermal fibroblasts (HDFs) were investigated in vitro. ADSCs and HDFs were isolated from healthy donors and flow cytometry was used for immunophenotype identification. ADSCs were co‑cultured with young or aged human dermal fibroblasts in Transwell plates, and control groups were established accordingly. Cellular proliferation was measured by an MTT assay. Type I collagen, matrix metalloproteinase‑1 (MMP‑1) and senescence-associated β‑galactosidase (SA‑β‑GAL) mRNA expression were measured by quantitative polymerase chain reaction. It was identified that ADSCs promoted proliferation of co‑cultured HDFs and induced increased expression of type I collagen and decreased expression of MMP‑1. The co‑cultured HDFs exhibited increased expression of SA‑β‑GAL. These results demonstrated that ADSCs improve fibroblast function through paracrine effects. The increased expression of SA‑β‑GAL indicated an accelerated aging process. It is proposed that ADSCs may improve fibroblast function, but not reverse the age status in vitro.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
How we grade evidenceBrowse all related research
Filter the research library by this study's title keywords, author, or publication year.