[Adipose tissue-derived adult stem cells transfected with the gene of hyperpolarization-activated cyclic nucleotide-gated ion channel 2 differentiated into pacemaker-like cells].
Li Y., Li B., Li Z., Zhang J., Zeng M.
Animal Study, published in Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi (2013) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi (2013)
- Country
- China
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 24011147
Abstract (original English)
To transfect rat adipose tissue-derived adult stem cells (ADSCs) with the pacemaker gene of human hyperpolarization-activated cyclic nucleotide-gated ion channel 2 (hHCN2), and observe its feasibility of functioning as pacemaker cells. First of all, healthy ADSCs were harvested and transfected with hHCN2 gene. Thereafter, we detected the expression of hHCN2 gene and protein by quantitative real-time PCR (qRT-PCR), Western blot analysis and immunofluorescence cytochemistry and measured the electrophysiological properties and recorded the inward currents using patch clamp techniques. In addition, we mixed and co-cultured the transfected ADSCs and rat cadiomyocytes to observe the influence on the spontaneous beating rate of cadiomyocytes. Compared with the control ADSCs, the transfected ADSCs expressed the higher level of hHCN2 gene and protein(P<0.05), and they could produce pacemaker current. When co-cultured with rat ventricular myocytes, they could accelerate the spontaneous beating rate of the ventricular myocytes(P<0.05). After transfected with hHCN2 gene, ADSCs can work as pacemaker-like cells, which provides materials for the research of biological pacemakers.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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