Advancing urine-derived stem cells: Cryopreservation validation and sex-specific metabolism
Afonso GJM., Cavaleiro C., Nunes SM., Pereira F., Oliveira PJ., Valero J.
Laboratory Study, published in Eur J Clin Invest (2026) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Eur J Clin Invest (2026)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 41587925
- PMCID
- PMC12834848
- DOI
- 10.1111/eci.70172
- Citations
- 1
Abstract (original English)
Background Urine-derived stem cells (UDSC) are an emerging, non-invasive source of human stem cells combining easy collection, broad accessibility and high patient compliance with multilineage differentiation capacity. However, key gaps remain in UDSC research, particularly in understanding sex-related differences and the lack of a validated cryopreservation protocol, a critical aspect for primary cells, given their variability in colony formation, proliferation rates and experimental timing. To address these limitations, this study aimed to establish, for the first time, a reliable protocol for UDSC cryopreservation and to explore potential sex-related differences, with a specific focus on glycolysis and mitochondrial respiration. Methods UDSC were isolated from urine samples of healthy donors (aged 27-50, 4 males and 4 females), cultured in 1:1 DMEM:KSFM supplemented with 10% fetal bovine serum and cryopreserved at passages 2-4 using the same medium with the sole addition of 5% dimethyl sulfoxide. Cells were evaluated for viability, apoptosis/necrosis, metabolic profile and multilineage differentiation potential. Comparisons were performed based on donor sex, as well as before and after cryopreservation. Results Male- and female-derived UDSC displayed no significant differences in viability and cell death or metabolic profile. Moreover, supervised and unsupervised machine lea
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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