Alterations of matrisome gene expression in multipotent mesenchymal stromal cells under physiological hypoxia in vitro.
Matveeva D., Andreeva E., Rudimova Y., Gornostaeva A., Yakubets D., Andrianova I.
Laboratory Study, published in Tissue Cell (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
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- Study type
- Laboratory Study
- Journal
- Tissue Cell (2025)
- Country
- Scotland
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 40753731
- DOI
- 10.1016/j.tice.2025.103064
Abstract (original English)
Low O 2 level (physiological hypoxia) is an important physical parameter in local tissue niches of multipotent mesenchymal stromal cells (MSCs). Hypoxia preconditioning is actively applied in cell therapy and regenerative medicine protocols. In the present study, the effect of physiologic hypoxia in vitro (5 % O 2 ) on the extracellular matrix of MSCs from the stromal-vascular fraction of human adipose tissue was investigated. Compared to standard cell culture conditions (20 % O 2 ), the genes encoding structural and regulatory proteins of the extracellular matrix (matrisome) were differentially expressed in MSCs under physiologic hypoxia. There was a significant downregulation of genes coding structural glycoproteins (COMP, ELN) in the core matrisome and upregulation of genes encoded matrisome-associated proteins with pro-migratory (CXCL12) and antioxidant (SRPX, SERPINF1) functions. At different O 2 levels, there were no significant differences in immunocytochemically identified the core matrisome proteins: collagen I, fibronectin, osteonectin, versican, nor in the expression of the corresponding genes. Meanwhile, variations in packaging patterns of the fibrils were, however, demonstrated using scanning electron microscopy. Under 5 % O 2 the activities of the soluble matrix metalloproteinases MMP-1 and MMP-2 were reduced. Thus, physiological hypoxia modulates the matrisome pr
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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