An alternative method for harvest and processing fat grafts: an in vitro study of cell viability and survival.
Gonzalez AM., Lobocki C., Kelly CP., Jackson IT.
Animal Study with a reported sample of 5, published in Plast Reconstr Surg (2007) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Plast Reconstr Surg (2007)
- Country
- United States
- Reported sample size
- 5
- Source database
- PubMed
- PMID
- 17572577
- DOI
- 10.1097/01.prs.0000264401.19469.ad
Abstract (original English)
Adipocyte viability has been emphasized as essential for fat graft survival. There is no universal agreement on the methodology for handling fat grafts. Two different methods of fat harvesting and techniques of tissue processing were compared by assessing cell viability, damage, and growth in vitro. Fat was harvested from Zucker rats (n = 5) using (1) a 3-mm liposuction cannula with a 60-cc syringe (right side) or (2) a 2-mm blunt needle with a 10-cc syringe adapted to a fine-needle aspiration apparatus (left side). Tissues were then processed by decantation or cotton towel drying. Five samples for each of the four techniques were studied. Fat samples were processed for culture, and adipocytes and preadipocytes were plated in culture medium and expanded in vitro. Cell viability was assessed using cell counts, the MTT proliferation assay, G3PDH activity, and Oil Red O stain. Method 1 exerted significantly higher pressure (p = 0.009) than method 2 (643 +/- 2.5 versus 537 +/- 13.6 mm Hg). A larger oil layer was apparent with method 1 (1.11 +/- 0.29 g) than with method 2 (0.56 +/- 0.28 g). In addition, the highest number of viable preadipocytes was obtained using method 2B (p = 0.017). In culture, preadipocytes plated in 4F differentiation medium started to differentiate after 1 week, while those in Dulbecco's modified Eagle's medium/F12 with serum proliferated but did not differen
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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