Amniotic membrane-encapsulated chitosan-lecithin nanoparticles promote the regenerative potential of mesenchymal stromal cells and fibroblasts.
Abou-Shanab AM., Fytory M., Shouman S., Atta D., Khairy A., Salah RA.
Laboratory Study, published in Nanoscale Adv (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Nanoscale Adv (2025)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 40657618
- DOI
- 10.1039/d5na00222b
Abstract (original English)
The human amniotic membrane (hAM) is a promising therapeutic source in regenerative medicine due to its rich content of growth factors and bioactive molecules. Its clinical application, however, has remained limited by challenges in controlled delivery and compositional complexity. To address these issues, we developed a nanoparticle-based formulation (NF-hAM) to encapsulate hAM proteins and enhance their targeted delivery to mesenchymal stem cells (MSCs) and fibroblasts. NF-hAM was synthesized using soy lecithin and chitosan, forming spherical nanoparticles with well-defined morphology. Physicochemical characterization confirmed successful protein encapsulation and nanoparticle stability. In vitro studies demonstrated that NF-hAM was biocompatible and significantly promoted the proliferation of bone marrow-derived MSCs (BMSCs), adipose-derived MSCs (AMSCs), and human skin fibroblasts (HSFs). In HSFs, NF-hAM also induced cytoskeletal remodeling, suggesting enhanced cellular activity and functional responsiveness. Additionally, NF-hAM enhanced the osteogenic and adipogenic differentiation potential of BMSCs. Metabolic analyses further revealed that NF-hAM stimulated glycolytic activity, a key metabolic pathway associated with self-renewal and proliferation. Our findings highlight the potential of NF-hAM as a therapeutic nanomedicine for regenerative medical applications.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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