Analysis of c-erb A RNA expression in the triiodothyronine-sensitive Ob 17 preadipocyte cell line.
Teboul M., Torresani J.
Animal Study, published in J Recept Res (1993) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- J Recept Res (1993)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 8385217
- DOI
- 10.3109/10799899309073695
Abstract (original English)
The protooncogenes erb A alpha and beta encode, in the rat, three functional thyroid hormone receptors (erb A alpha 1, beta 1 and beta 2), and two isoforms (erb A alpha 2, alpha 3) that do not bind triiodothyronine (T3). We previously reported on a mouse preadipocyte cell line (Ob 17) which was found to be sensitive to thyroid hormones and retinoic acid. Using antibodies or cDNA probes, we reported that the c-erb A products are mainly of the alpha-type in these cells. We also showed that the thyroid hormone receptors/c-erb A products are down-regulated moderately by T3 and strongly by retinoic acid added to the culture medium. In this work, different c-erb A subtypes are identified in this mouse cell line. Using couples of oligonucleotides known to be specific of each rat c-erb A subtype, we could detect the presence of alpha 1, alpha 2 and beta 1-type transcripts. We also detected Rev transcripts of a Reverse erb A alpha gene present in the rat at the c-erb A alpha locus and that contains a region complementary to the c-erb A alpha 2-specific region. These transcripts were identified by PCR amplification from cell cDNAs and in Northern analyses using the corresponding PCR-designed c-erb A probes. All the detected transcripts were found to be down-regulated by retinoic acid.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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