Analysis of the Regeneration Ability of Adipose-Derived Schwann Cells for Sciatic Nerve Defects.
Chen L., Qi Z.
Animal Study, published in Ann Plast Surg (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Ann Plast Surg (2025)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 40498972
- DOI
- 10.1097/SAP.0000000000004338
Abstract (original English)
Background Schwann cells (SC) are foremost seed cells in peripheral nerve repair. Our previous research had succeeded in culturing a new source of SC from tiny nerve fibers of subcutaneous adipose tissue (A-SC) and proved their ability to form myelin sheaths. However, little is known regarding the quality and the microstructure of regenerated nerves after A-SC transplantation. Methods To further explore the efficacy of A-SC on nerve regeneration, A-SC, sciatic nerve derived Schwann cells (SN-SC) and adipose-derived stem cells (ADSC) from CAG-EGFP transgenic mice were harvested and separately injected into the nerve conduit to repair sciatic nerve defects in wild-type mice. The regenerated nerves were studied 3 and 6 months after surgery. HE staining was used to roughly observe the histological features of regenerated nerves. Immunofluorescence staining was used to evaluate the quality of regenerated axons. Transmission electron microscopy was conducted to observe the microstructure and assess the mature level of regenerated nerves. Results Hematoxylin and eosin staining and immunofluorescence staining showed that, in A-SC group, regenerated nerves exhibited better organized structures and the number of regenerated nerve fibers was significantly higher than that in ADSC group but a bit lower than that in SN-SC group. In addition, transmission electron microscopy revealed that mi
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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