Biocompatible nanofibrous scaffolds incorporating titanium dioxide and melatonin to enhance osteogenesis in ADMSCs.
MohammadEbrahim N., Khalili M., Rad I., Mehrabadi M., Noghlebari NA., Akhlaghpasand M.
Laboratory Study, published in Naunyn Schmiedebergs Arch Pharmacol (2026) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Naunyn Schmiedebergs Arch Pharmacol (2026)
- Country
- Germany
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 42082728
- DOI
- 10.1007/s00210-026-05155-6
Abstract (original English)
Large bone defects remain a significant clinical challenge due to the limitations of autografts and allografts, prompting interest in bioactive scaffolds for bone tissue engineering. This study aimed to develop and evaluate electrospun nanofibrous scaffolds composed of polycaprolactone (PCL) and polylactic acid (PLA), incorporating titanium dioxide nanoparticles (TiO₂) and melatonin (Mel), to enhance their osteogenic differentiation potential. PCL/PLA, PCL/PLA/TiO₂, PCL/PLA/Mel, and PCL/PLA/TiO₂/Mel scaffolds were fabricated via electrospinning and characterized for morphology, fiber diameter, and mechanical properties. Biological performance was evaluated using adipose-derived mesenchymal stem cells (ADMSCs) through MTT assay, calcium content, alkaline phosphatase (ALP) activity, and osteogenic gene expression. TEM confirmed uniform TiO₂ nanoparticle distribution without agglomeration. All scaffolds exhibited continuous cylindrical fibers without defects. TiO₂ incorporation reduced tensile strength but increased elongation, while melatonin enhanced tensile strength. MTT assays confirmed biocompatibility and higher proliferation in TiO₂- and Mel-containing scaffolds. Calcium content and ALP activity were significantly higher in TiO₂- and/or Mel-modified scaffolds, with the PCL/PLA/TiO₂/Mel group showing the most significant osteogenic differentiation potential. Gene expression
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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