Bioinspired osteoblast-imprinted piezoelectric PDMS/BaTiO 3 nanocomposites accelerate the osteogenic differentiation of adipose-derived stem cells under mechanical stimulation.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

In this study, we propose a new cell culture substrate that can accelerate the osteogenic differentiation of adipose-derived mesenchymal stem cells (ADSCs) by exploiting the synergistic effects of cellular imprinting and mechanoelectrical stimulation. These substrates were prepared by imprinting osteoblast footprints onto the surface of nanocomposites based on polydimethylsiloxane (PDMS) filled with 20 wt% tetragonal barium titanate (BaTiO 3 ) piezoelectric nanoparticles. In order to assess the osteoinductive potential of the substrates, in vitro cellular assays were carried out under dynamic mechanical stimulations. The results of cellular analysis showed that the osteoblastic imprints were highly influential in the regulation of ADSCs' functions and osteogenic differentiation. In substrates based on pristine PDMS, the surface imprints simulated the micro/nano topographies of natural bone extracellular matrix (ECM) that led to the enhancement of cell-substrate interactions and direction of cellular fate. Meanwhile, the incorporation of BaTiO 3 nanoparticles into the imprinted samples further enhanced the focal adhesion, extension, and proliferation of ADSCs due to the accumulation of electric charges and elevation of surface electric potential. Extensive Ca 2+ ion deposition and acceleration of bone mineralization were also reported for the ADSCs cultured on the PDMS/20BaTiO 3

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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