Biological and Metabolomic Characterization of Human Dermal Fibroblasts and Mesenchymal Stem Cells Derived from Human Dental Pulp and Adipose Tissue: a Pilot Comparative Study.
Hatoková Z., Baďurová B., Kertys M., Žideková N., Evinová A., Kotúľová L.
Cohort Study, published in Stem Cell Rev Rep (2025) — summary generated from the PubMed abstract.
Early human evidence such as case series or small samples is exploring possible benefits.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Cohort Study
- Journal
- Stem Cell Rev Rep (2025)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 40928693
- PMCID
- PMC12504344
- DOI
- 10.1007/s12015-025-10970-0
- Citations
- 1
Abstract (original English)
Background Several studies have suggested that adult human dermal fibroblasts (HDFa) may be a potential alternative source to mesenchymal stem cells for cell therapies. This study aims to characterize HDFa, adipose-derived stem cells (ADMSCs) and dental pulp stem cells (DPSCs) to investigate their proliferation, differentiation potential, mitochondrial respiration, and metabolomic profile. We identified molecules and characteristics that would differentiate MSCs from different sources or confirm their uniformity. Methods Differentiation was induced using osteogenic and adipogenic differentiation media. Proteins specific to each differentiation process were monitored by immunofluorescence staining. High-resolution respirometry and targeted metabolomic analysis using the AbsoluteIDQ ® p180 kit (Biocrates Life Science) were applied to identify the essential properties of the studied cells. Results HDFa cells, ADMSCs, and DPSCs demonstrated morphological characteristics of mesenchymal stem cells (MSCs). In general, DPSCs and HDFa showed significantly higher proliferation than ADMSCs. Osteogenic and adipogenic capacities were similar for all cell origins after 21 days, but ADMSCs exhibited earlier calcium deposit formation. FoxO1 and adiponectin as osteogenic and adipogenic-related proteins confirmed differentiation processes. High-resolution respirometry and metabolomic analysis sh
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
Evidence level
Early human evidence such as case series or small samples is exploring possible benefits.
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