Biomimetic integration of functionally controlled modular tissue building blocks for engineering 3D vascularized adipose tissue.
Lee S., Lee J., Kwon H., Shin H.
Animal Study, published in Bioact Mater (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Bioact Mater (2025)
- Country
- China
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 40046010
- PMCID
- PMC11880769
- DOI
- 10.1016/j.bioactmat.2025.02.024
- Citations
- 4
Abstract (original English)
Adipose tissue is highly vascularized, which is crucial for homeostasis and energy storage. Current efforts to engineer 3D vascularized adipose tissue in vitro typically involve co-culturing adipocytes and endothelial cells, but adipogenic differentiation often suppresses endothelial function. In this study, we propose a novel approach to reconstruct vascularized adipose tissues in vitro by effectively coupling adipogenesis and vasculogenesis. First, we developed adipo-inductive nanofibers (ID/F@INS) that contain indomethacin and insulin. The in vitro adipogenesis of human adipose-derived stem cells (hADSCs) in general medium was significantly enhanced in adipogenic spheroids (AS) prepared with hADSCs and ID/F@INS, which were encapsulated in a gelatin methacryloyl (GelMA) hydrogel. To further replicate clustering during de novo adipogenesis, we generated AS of varying sizes and found that larger spheroids exhibited markedly greater adipogenesis than smaller ones. At the same time, we used hADSCs and human umbilical vein endothelial cells to generate vascular spheroids (VS). The biomimetic integration of AS and VS within GelMA hydrogels enabled us to investigate the interactions between de novo adipogenesis and vascularization. The integration of the two types of spheroids (VS:AS ratio of 2:1) significantly improved vascular network formation, indicating the concurrent stimulati
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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