Bone regeneration using adipose derived stem cell spheroids within 3D printed scaffolds in a rabbit radial defect model.
Chae Y., Jang K., Lee S., Kim YH., Jin S., Shim KM.
Animal Study, published in Sci Rep (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Sci Rep (2025)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 41402377
- PMCID
- PMC12711910
- DOI
- 10.1038/s41598-025-25581-5
- Citations
- 1
Abstract (original English)
Bone tissue is generally resilient and can self-heal, but critical-size defects (CSDs) with complex geometries cannot be repaired without clinical intervention. Customized scaffolds developed using three-dimensional (3D) printing techniques can effectively repair complex-shaped CSDs. Adipose-derived stem cells (ADSCs), a type of mesenchymal stem cell (MSC), can differentiate into osteoblasts and exhibit osteoinductive properties. However, ADSC-single cells fabricated via two-dimensional (2D) monolayer cultures have limitations in maintaining cell survival and function over time. Unlike 2D monolayer cultures, ADSC-spheroids fabricated via 3D spheroid cultures can overcome this limitation by increasing the survival of ADSCs and enhancing their in vivo osteogenic capacity. This study aimed to evaluate the potential of a synergistic strategy of ADSC-spheroids within a 3D-printed scaffold made of polycaprolactone/hydroxyapatite (PCL/HA) in bone regeneration. In vitro experiments demonstrated that ADSC-spheroids promoted mineralization in 3D-printed scaffolds. Radiographs and histological analysis performed at eight weeks post-implantation in in vivo experiments using a rabbit radial defect model showed successful bone regeneration in the group containing ADSC-spheroids within the PCL/HA scaffold. These results suggest that the synergistic strategy of incorporating ADSC-spheroids int
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
How we grade evidenceBrowse all related research
Filter the research library by this study's title keywords, author, or publication year.