Bovine Placental Cotyledon-Derived Hydrogel: Methodology to Produce a Substrate for Tissue Engineering.
Candelária RAQ., Cordeiro IS., Miglino MA., Barreto RSN.
Animal Study with a reported sample of 10, published in Tissue Eng Part C Methods (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Tissue Eng Part C Methods (2025)
- Country
- United States
- Reported sample size
- 10
- Source database
- PubMed
- PMID
- 40657703
- DOI
- 10.1177/19373341251360957
Abstract (original English)
Bioengineering aims to develop biomaterials that closely mimic the native extracellular matrix (ECM) to support tissue regeneration. This study presents a detailed protocol for producing hydrogels derived from decellularized bovine placental cotyledons. Bovine placentas at 4-5 months of gestation ( n = 10) were subjected to vascular perfusion with increasing concentrations of sodium dodecyl sulfate (0.01-1%) and Triton X-100 (1%), which effectively removed cellular components. Decellularization efficacy was confirmed by histological (hematoxylin and eosin and 4',6-diamidino-2-phenylindole [DAPI] staining), molecular, and structural analyses, including residual genomic DNA quantification averaging 9.1 ng/mg of dry tissue. The ECM scaffolds were enzymatically digested using 0.1% (w/v) pepsin in 0.01 M HCl and reconstituted with sodium alginate at concentrations of 5%, 8%, 10%, and 12% (w/v). Crosslinking was achieved with 1% calcium chloride. Among the tested formulations, hydrogels containing 12% alginate demonstrated greater mechanical stability and preserved three-dimensional architecture, including interconnected porosity, as evidenced by scanning electron microscopy. Cytocompatibility was evaluated by culturing canine adipose-derived mesenchymal stem cells on both decellularized biomaterials and hydrogels. DAPI staining revealed nuclei after 7 and 25 days of culture, indicat
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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