Bovine Stearoyl-CoA Desaturase 1 Promotes Adipogenesis by Activating the PPARγ Receptor.
Zhang Y., Zheng Y., Wang X., Qiu J., Liang C., Cheng G.
Animal Study, published in J Agric Food Chem (2020) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- J Agric Food Chem (2020)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 33052678
- DOI
- 10.1021/acs.jafc.0c05147
- Citations
- 16
Abstract (original English)
Stearoyl-CoA desaturase 1 (SCD1) is a rate-limiting enzyme that mainly catalyzes the saturated fatty acids (SFAs) into the monounsaturated fatty acids (MUFAs). The expression level of SCD 1 is positively correlated with the marbling score. However, the functional mechanism of SCD 1 in adipogenesis is still unclear. In this study, we identified SCD 1 as highly expressed in subcutaneous and visceral fat, peaking at 2 days after differentiation in bovine stromal vascular fraction (SVF) cells. When the SCD 1 was overexpressed in bovine SVF cells, lipid droplets accumulation was increased from 142.46 ± 21.77 to 254.89 ± 11.75 μg/mg ( P < 0.01). Further, the expression levels of FABP 4, FASN , and ACC α were increased ( P < 0.01), while the expression of PPAR γ or C/EBP α was not changed at mRNA or protein level ( P > 0.05). Dual-luciferase reporter assay showed that the activity of the PPARγ receptor was enhanced by 3.69 times ( P < 0.01). Moreover, the contents of palmitoleate (C16:1) and oleate (C18:1) were significantly increased ( P < 0.05). Furthermore, 100 μM exogenous oleate increased the lipid accumulation by 22.28 times ( P < 0.01). These results suggest that oleate is probably a strong ligand of the PPARγ receptor to enhance adipogenesis.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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