Brown adipose-derived mesenchymal stromal cells improve sepsis-induced multiorgan dysfunction more than white adipose cells in obese rats.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Background/aims Sepsis remains a leading cause of mortality worldwide and is driven by a dysregulated host response to infection. Mesenchymal stromal cells (MSCs) derived from white adipose tissue (WAT) have shown therapeutic potential in sepsis; however, the properties of brown adipose tissue (BAT)-derived MSCs remain poorly characterized, particularly in obesity, a condition known to alter MSC phenotype and function. We compared the therapeutic efficacy of MSCs isolated from brown versus white adipose depots of obese donors in a clinically relevant model of sepsis in obese rats. Methods Obesity was induced in Wistar rats through a high-fat diet. MSCs were isolated from brown and white adipose tissue and characterized by plastic adherence, surface marker expression, and gene expression profiling. Sepsis was induced by cecal ligation and puncture (CLP). Twenty-four hours after CLP, animals received saline, WAT-MSCs, or BAT-MSCs (1 × 10⁶ cells). Primary outcomes were 48-h survival and bacterial burden. Secondary outcomes included blinded histological assessment and molecular analyses of lung, kidney, and liver injury, focusing on inflammatory cytokines, fibrotic signaling, and oxidative stress. Results Both MSC populations met established characterization criteria. BAT-MSCs exhibited higher baseline expression of anti-inflammatory and antioxidant genes. In obese septic rats, tre

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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