Browning effect of LncRNA00602 in Ad36-induced differentiated adipocytes.
Gao J., Meng X., Jiao Y., Abudukeremu A., Nuermaimaiti N., Ma W.
Laboratory Study, published in Medicine (Baltimore) (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Medicine (Baltimore) (2025)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 40859510
- PMCID
- PMC12384816
- DOI
- 10.1097/MD.0000000000043816
Abstract (original English)
Long noncoding RNAs (LncRNAs) play critical roles in regulating gene expression and cellular processes, including adipocyte differentiation. LncRNA00602 is upregulated in adipose tissue. Therefore, we aimed to investigate the potential role of LncRNA00602 in adenovirus type 36 (Ad36)-induced adipocyte browning. Human adipose-derived stem cells were induced into adipocytes by Ad36, and were divided into the control and LncRNA00602 knockdown groups. Lipid droplets and mitochondria were stained with BODIPY and Mito Tracker Red, respectively. The binding of LncRNA00602 to upstream frameshift 1 (UPF1) was detected by RNA-immunoprecipitation. We used silver staining, co-immunoprecipitation, and mass spectrometry to detect and identify the protein complexes bound to UPF1. The control and caveolin-1 (CAV-1) knockdown groups were set up. The mRNA and protein levels were detected by real-time quantitative PCR and Western Blot. On days 2 and 4, compared with the control group, the lipid droplet area of adipocytes in the LncRNA00602 knockdown group was larger, whereas the mitochondrial fluorescence intensity was lower. We observed significant reductions in the mRNA and protein expression levels of ATP5O and COX5B in the LncRNA00602 knockdown group. LncRNA00602 could bind to UPF1. The proteins bound to UPF1 were involved in the nonsense-mediated mRNA decay pathway and lipid metabolism. Addi
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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