C-C chemokine ligand 5 from women subcutaneous adipose tissue has a central role in vascular aging.

Early human evidence such as case series or small samples is exploring possible benefits.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

BACKGROUND: Aging is associated with adipose tissue alterations, oxidative stress, and fibrosis and the onset of cardiometabolic complications. While it has been shown that perivascular adipose tissue (PVAT) contributes to vascular damage, the involvement of subcutaneous adipose tissue (SCAT) - particularly through its secretory activity - in vascular aging remains poorly understood. Previously, we have demonstrated that human adipose-derived stromal cells (ASCs) from the SCAT of aged women display senescence and oxidative stress. We hypothesized that the ASC secretome contributes to the onset of endothelial dysfunction, an early stage of vascular aging. METHODS: We prepared conditioned media from ASCs isolated from SCAT of healthy young (< 25y) or aged (> 60y) women. The ASCs secretome was analyzed and added on human coronary artery endothelial cells (HCAECs). Using clinical cohorts, we evaluated the expression of C-C-chemokine-ligand-5 (CCL5)/Regulated upon-Activation-Normally-T-expressed-and-secreted (RANTES) in adipose tissue of individuals with coronary heart disease. RESULTS: The secretome of aged-donor ASCs induced endothelial cell dysfunction in HCAEC, as evidenced by lower nitric oxide production, higher oxidative stress, senescence, and a pro-adherent phenotype. Aged-donor ASCs also favored the endothelial-to-mesenchymal transition, characterized by the higher express

Evidence level

Early human evidence such as case series or small samples is exploring possible benefits.

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