Calcium Peroxide-Containing Polydimethylsiloxane-Based Microwells for Inhibiting Cell Death in Spheroids through Improved Oxygen Supply.
Mizukami Y., Takahashi Y., Shimizu K., Konishi S., Takakura Y., Nishikawa M.
Laboratory Study, published in Biol Pharm Bull (2021) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Biol Pharm Bull (2021)
- Country
- Japan
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 34602554
- DOI
- 10.1248/bpb.b21-00269
- Citations
- 4
Abstract (original English)
Multicellular spheroids are expected to be used for in vivo-like tissue models and cell transplantation. Microwell devices are useful for the fabrication of multicellular spheroids to improve productivity and regulate their size. However, the high cell density in microwell devices leads to accelerated cell death. In this study, we developed O 2 -generating microwells by incorporating calcium peroxide (CaO 2 ) into polydimethylsiloxane (PDMS)-based microwells. The CaO 2 -containing PDMS was shown to generate O 2 for 3 d. Then, CaO 2 -containing PDMS was used to fabricate O 2 -generating microwells using a micro-molding technique. When human hepatocellular carcinoma (HepG2) spheroids were prepared using the conventional microwells, the O 2 concentration in the culture medium reduced to approx. 67% of the cell-free level. In contrast, the O 2 -generating microwells maintained O 2 at constant levels. The HepG2 spheroids prepared using the O 2 -generating microwells had a larger number of live cells than those prepared using the conventional microwells. In addition, the O 2 -generating microwells rescued hypoxia in the HepG2 spheroids and increased cell viability. Lastly, the O 2 -generating microwells were also useful for the preparation of multicellular spheroids of other cell types (i.e., MIN6, B16-BL6, and adipose-derived stem cells) with high cell viability. These results showe
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
How we grade evidenceBrowse all related research
Filter the research library by this study's title keywords, author, or publication year.