Carboxymethyl Chitosan-Functionalized Polyaniline/Polyacrylonitrile Nano-Fibers for Neural Differentiation of Mesenchymal Stem Cells.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Electroconductive scaffolds based on polyaniline (PANi)/polyacrylonitrile (PAN) were fabricated and surface-functionalized by carboxymethyl chitosan (CMC) as efficient scaffolds for nerve tissue regeneration. The results of scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, and water contact angle measurement approved the successful fabrication of CMC-functionalized PANi/PAN-based scaffolds. Human adipose-derived mesenchymal stem cells (hADMSCs) were cultured on the scaffolds for 10 d in the presence or absence of β-carotene (βC, 20 µM) as a natural neural differentiation agent. The MTT and SEM results confirmed the attachment and proliferation of hADMSCs on the scaffolds. The expression of MAP2 at the mRNA and protein levels showed the synergic neurogenic induction effect of CMC-functionalization and βC for hADMSCs on the scaffolds. The CMC-functionalized nanofibrous PANi/PAN-based scaffolds are potential candidates for nerve tissue engineering.

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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