CD11c<sup>-</sup>MHC2<sup>low</sup> Macrophages Are a New Inflammatory and Dynamic Subset in Murine Adipose Tissue
Wetzels S., Bijnen M., Wijnands E., van de Gaar J., Tan A., Coort S.
Animal Study, published in Immunometabolism (2020) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Immunometabolism (2020)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 34987865
- PMCID
- PMC7612162
- DOI
- 10.20900/immunometab20200015
- Citations
- 1
Abstract (original English)
Background The prevalence of obesity is rising and leads to increased morbidity and mortality. Adipose tissue inflammation, due to accumulation and activation of adipose tissue macrophages (ATMs), is a key driver of this phenomenon. Macrophages are heterogeneous cells, adapting quickly to the microenvironment, resulting in so-called M1 or M2 macrophages. In this study, we describe the dynamics and inflammatory properties of a newly identified ATM subset in obese mice. Methods LDLR -/- mice received a high fat diet (HFD) for 5 weeks or 16 weeks to induce obesity. Adipose tissues were isolated and immune cell subsets were analyzed with flow cytometry or microarray analysis. Bone marrow transplantation (BMT) using CD45.1 and CD45.2 LDLR -/- mice was performed to determine ATM origin. Results Upon HFD, there is a massive increase of ATM subsets in the adipose tissue. CD11c - M2 ATMs could be subdivided based on their MHC2 expression into CD11c - MHC2 high ATMs and previously unidentified CD11c - MHC2 low ATMs. CD11c - MHC2 low ATMs accumulated very rapidly after 10 days of HFD, after which they increased even further with prolonged HFD. Microarray data showed that CD11c - MHC2 low ATMs resembled CD11c - MHC2 high ATMs in the steady state, but became more inflammatory during development of obesity. In vitro stimulation of bone marrow-derived macrophages with palmitate, abundantly pr
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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