CD146 (MCAM) in human cs-DLK1 - /cs-CD34 + adipose stromal/progenitor cells.

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

To precisely characterize CD146 in adipose stromal/progenitor cells (ASCs) we sorted the stromal vascular faction (SVF) of human abdominal subcutaneous white adipose tissue (sWAT) according to cell surface (cs) expression of CD146, DLK1 and CD34. This test identified three main SVF cell populations: ~50% cs-DLK1 - /cs-CD34 + /cs-CD146 - ASCs, ~7.5% cs-DLK1 + /cs-CD34 dim/+ /cs-CD146 + and ~7.5% cs-DLK1 + /cs-CD34 dim/+ /cs-CD146 - cells. All cells contained intracellular CD146. Whole mount fluorescent IHC staining of small vessels detected CD146 + endothelial cells (CD31 + /CD34 + /CD146 + ) and pericytes (CD31 - /CD34 - /CD146 + ASCs). The cells in the outer adventitial layer showed the typical ASC morphology, were strongly CD34 + and contained low amounts of intracellular CD146 protein (CD31 - /CD34 + /CD146 + ). Additionally, we detected wavy CD34 - /CD146 + and CD34 dim /CD146 + cells. CD34 dim /CD146 + cells were slightly more bulky than CD34 - /CD146 + cells. Both CD34 - /CD146 + and CD34 dim /CD146 + cells were detached from the inner pericyte layer and protruded into the outer adventitial layer. Cultured early passage ASCs contained low levels of CD146 mRNA, which was expressed in two different splicing variants, at a relatively high amount of the CD146-long form and at a relatively low amount of the CD146-short form. ASCs contained low levels of CD146 protein, which co

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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