A cell-laden methacrylated chitosan-based photocrosslinkable hydrogel for bone tissue engineering and its in vitro structural and biological characterization.
Samani S., Nazbar A., Vasei M., Bonakdar S., Azami M.
Animal Study, published in Sci Rep (2026) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Sci Rep (2026)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 41484309
- PMCID
- PMC12856020
- DOI
- 10.1038/s41598-025-34127-8
Abstract (original English)
The treatment of extensive bone defects involves the use of multicomponent scaffolds where different components can be precisely controlled according to specific conditions. Therefore, hybridizing biomaterials within cell-laden osteogenic bioscaffolds provides promising opportunities for clinical applications. In this study, a cell-laden, photocrosslinkable hydrogels composed of methacrylated chitosan (MECs) and silk fibroin (SF) fibers was developed to induce osteogenesis, and its structural and biological properties was investigated. SF fibers were mineralized with a hydroxyapatite (HAp) layer using a modified alternate soaking followed by heat treatment. Optimal photocrosslinking conditions were determined using the Taguchi method. SF fibers were incorporated into MECs at varying concentrations, and the resulting hydrogel structure was assessed with and without fibers under different ionic conditions. Adipose-derived stem cells (ADSCs) were encapsulated into hydrogels, and their morphology, viability, and osteogenic gene expression were analyzed. FTIR, XRD, and SEM confirmed successful mineralization of SF, with heat treatment enhancing HAp crystallinity. SF addition effectively prevented hydrogel shrinkage and promoted a porous structure due to fiber enrichment and double crosslinking. Encapsulated ADSCs remained viable after 14 days, and mineralized fibers significantly up
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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