Cell type specific differences in transcriptome profiles of adipose derived stem cells and vaginal fibroblasts in patients with pelvic organ prolapse.
Tchoukalova YD., Nair AA., Chen X., Zhang N., Myers CE., Badreldin A.
Prospective Study, published in Gene (2025) — summary generated from the PubMed abstract.
Early human evidence such as case series or small samples is exploring possible benefits.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Prospective Study
- Journal
- Gene (2025)
- Country
- Netherlands
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 39814191
- DOI
- 10.1016/j.gene.2025.149230
- Citations
- 1
Abstract (original English)
This study examined the molecular phenotypes of adipose-derived stem cells (ASCs) and vaginal fibroblasts (VFBs) and assessed whether pelvic organ prolapse (POP) affects their biological properties. We performed RNA sequencing of paired ASCs and VFBs from six patients with POP and six controls (CTRL). The transcriptomes of POP and CTRL in either ASCs or VFBs were compared (DESeq2, false discovery rate (FDR) < 0.05) to identify differentially expressed genes (DEGs). The transcriptomes of VFBs were compared between POP and CTRL (non-adjusted p < 0.01) followed by Ingenuity Pathway Analysis on DEGs considering that pathways with FDR < 0.05 could be pathogenic. We also performed a pairwise comparison after combining the gene expression data of POP and CTRL for ASCs and VFBs to identify cell type specific DEGs and analyzed the functional associations among them (STRING platform). We found no DEGs between POP and CTRL in ASCs and VFBs. Less stringent statistical analysis of VFBs transcriptome showed 23 genes with higher and 29 genes with lower expression in POP compared to CTRL. Among the latter were five genes involved in the synaptogenesis pathway found to be significant. We were only able to validate POP related differences for very low density receptor (VLDLR). We found 508 DEGs with 4-fold difference between ASCs and VFBs (both POP and CTRL groups combined for each cell type) wh
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
Evidence level
Early human evidence such as case series or small samples is exploring possible benefits.
How we grade evidenceBrowse all related research
Filter the research library by this study's title keywords, author, or publication year.