Cellular Uptake of Nanoparticles is Regulated by Integrin-Based Adhesion to the Extracellular Matrix.
Joshi S., Naha A., Ene J., Holmes C., Li Y., Driscoll T.
Animal Study, published in ACS Appl Mater Interfaces (2026) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- ACS Appl Mater Interfaces (2026)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 41493775
- PMCID
- PMC12867119
- DOI
- 10.1021/acsami.5c18030
- Citations
- 1
Abstract (original English)
Therapeutic nanoparticle delivery is crucial for a variety of biomedical applications, such as immunizations, gene and drug delivery, tissue engineering, biomedical implant coating, and other regenerative medicine approaches. The uptake of therapeutic nanoparticles occurs through several endocytosis pathways. However, the uptake mechanism of nanoparticles delivered from a substrate is also regulated by cell-substrate interactions and the ability of cells to mechanosense their surrounding extracellular matrix (ECM). These cell-ECM interactions influence integrin signaling, focal adhesion formation, and cytoskeletal rearrangement to impact nanoparticle uptake. In this study, we investigated the role of ECM and ECM-mimetic coatings─collagen I (COL), fibronectin (FN), laminin (LM), hyaluronic acid (HA), and poly-l-lysine (PLL)─on the uptake of poly(lactic- co -glycolic acid) (PLGA) nanoparticles across three distinct cell types: NIH3T3 fibroblasts, primary rat adipose-derived stem cells (ASCs), and RAW264.7 macrophages, which displayed varying levels of integrin-based focal adhesion formation. Using a quartz crystal microbalance with dissipation (QCM-D) and ellipsometry, we thoroughly characterized ECM coatings, showing variations in coating thickness and mechanical properties. FN and COL coatings significantly enhanced cell proliferation, spreading, and focal adhesion formation, c
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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