Characterization of Adipocyte Precursor Cells (APCs) by Lineage Tracing, Flow Cytometry, and Cytometry Time of Flight (CyTOF).

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Flow cytometry and fluorescence-activated cell sorting (FACS) techniques have significantly advanced the characterization of adipocyte precursor cell (APC) populations. They allow immuno-phenotyping, quantification, and isolation of distinct populations, which critical for understanding adipose tissue development and homeostasis. Here, we describe the identification and purification of APCs using flow cytometry, FACS, and cytometry by time-of-flight (CyTOF), defined by previously established surface marker profiles. In addition, we describe the mouse models and whole adipose tissue visualization techniques that will enable us to characterize the cellular origin and plasticity of APCs.

Evidence level

Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.

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