The Characterization and Differential Analysis of m 6 A Methylation in Hycole Rabbit Muscle and Adipose Tissue and Prediction of Regulatory Mechanism about Intramuscular Fat
Luo G., Ai Y., Yu L., Wang S., Ren Z.
Laboratory Study, published in Animals (Basel) (2023) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Animals (Basel) (2023)
- Reported sample size
- —
- Source database
- Europe PMC
- PMID
- 36766336
- PMCID
- PMC9913852
- DOI
- 10.3390/ani13030446
- Citations
- 1
Abstract (original English)
N6-methyladenosine (m 6 A) widely participates in various life processes of animals, including disease, memory, growth and development, etc. However, there is no report on m 6 A regulating intramuscular fat deposition in rabbits. In this study, m 6 A modification of Hycole rabbit muscle and adipose tissues were detected by MeRIP-Seq. In this case, 3 methylases and 12 genes modified by m 6 A were found to be significantly different between muscle and adipose tissues. At the same time, we found 3 methylases can regulate the expression of 12 genes in different ways and the function of 12 genes is related to fat deposition base on existing studies. 12 genes were modified by m 6 A methylase in rabbit muscle and adipose tissues. These results suggest that 3 methylases may regulate the expression of 12 genes through different pathways. In addition, the analysis of results showed that 6 of the 12 genes regulated eight signaling pathways, which regulated intramuscular fat deposition. RT-qPCR was used to validate the sequencing results and found the expression results of RT-qPCR and sequencing results are consistent. In summary, METTL4 , ZC3H13 and IGF2BP2 regulated intramuscular fat by m 6 A modified gene/signaling pathways. Our work provided a new molecular basis and a new way to produce rabbit meat with good taste.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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