Characterization of human adipose tissue-derived stem cells with enhanced angiogenic and adipogenic properties.
Lauvrud AT., Kelk P., Wiberg M., Kingham PJ.
Prospective Study with a reported sample of 8, published in J Tissue Eng Regen Med (2016) — summary generated from the PubMed abstract.
Early human evidence such as case series or small samples is exploring possible benefits.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Prospective Study
- Journal
- J Tissue Eng Regen Med (2016)
- Country
- England
- Reported sample size
- 8
- Source database
- PubMed
- PMID
- 26833948
- DOI
- 10.1002/term.2147
- Citations
- 44
Abstract (original English)
Autologous fat grafting is a popular method for soft tissue reconstructions but graft survival remains highly unpredictable. Supplementation of the graft with the stromal vascular fraction (SVF) or cultured adipose tissue-derived stem cells (ASCs) can enhance graft viability. In this study we have examined the phenotypic properties of a selected population of cells isolated from ASCs, with a view to determining their suitability for transplantation into grafts. ASCs were isolated from the SVF of human abdominal fat (n = 8 female patients) and CD146 + cells were selected using immunomagnetic beads. The angiogenic and adipogenic properties of the positively selected cells were compared with the negative fraction. CD146 + cells expressed the immunophenotypic characteristics of pericytes. With prolonged in vitro expansion, CD146 - cells exhibited increased population doubling times and morphological signs of senescence, whereas CD146 + cells did not. CD146 + cells expressed higher levels of the angiogenic molecules VEGF-A, angiopoietin-1 and FGF-1. Conditioned medium taken from CD146 + cells significantly increased formation of in vitro endothelial cell tube networks, whereas CD146 - cells did not. CD146 + cells could be differentiated into adipocytes in greater numbers than CD146 - cells. Consistent with this, differentiated CD146 + cells expressed higher levels of the adipocyte m
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
Evidence level
Early human evidence such as case series or small samples is exploring possible benefits.
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