Chemical induced conversion of mouse fibroblasts and human adipose-derived stem cells into skeletal muscle-like cells.
Bansal V., De D., An J., Kang TM., Jeong HJ., Kang JS.
Animal Study, published in Biomaterials (2018) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Biomaterials (2018)
- Country
- Netherlands
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 30554025
- DOI
- 10.1016/j.biomaterials.2018.11.037
- Citations
- 21
Abstract (original English)
Use of stem cells in regenerative medicine holds great promise in treating people suffering from various otherwise incurable ailments. Direct conversion of somatic cells to other lineages thereby bypassing the intermediate pluripotent state has enormous applicability with respect to time requirement for conversion as well as safety issues. Among various approaches, chemical induced cell conversion is safe yet effective, and the use of small molecules has thus increased greatly in recent years in regenerative fields due to easy applicability, efficient scalability, and consistent reproducibility. Here we report a combination of small molecules capable of converting mouse fibroblasts into skeletal muscle-like cells (SMLCs) without requiring ectopic transcription factor expression. We observed that a combination of chemicals is necessary and sufficient to convert mouse fibroblast to SMLCs that have functional similarity to skeletal muscles. In addition, we also found that cytokines responsible for modulating several key signaling pathways enhance the maturation of converted SMLCs into multinucleated myocytes. Epigenetic analysis revealed that this conversion is accomplished by an epigenetic overhaul, followed by activation of key signal pathways responsible for activating skeletal specific loci. We further observed that human adipocyte-derived stem cells can be converted into SMLC
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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