Chondrogenic potential of progenitor cells derived from human bone marrow and adipose tissue: a patient-matched comparison.

Early human evidence such as case series or small samples is exploring possible benefits.

• Level A · Stronger Clinical Evidence
• Level B · Emerging clinical evidence with positive signals
• Level C · Early human research exploring benefits
• Level D · Scientific groundwork from lab and animal studies
• Emerging · Emerging topic under active research

Abstract (original English)

Stem cell-based tissue engineering represents a possible alternative for the repair of cartilage defects. Both bone marrow and adipose tissue contain pluripotential cells capable of chondrogenesis. This study was a qualitative and quantitative comparison of the chondrogenic potential of progenitor cells isolated from bone marrow aspirates and adipose tissue. Bone marrow aspirates (BM) and matching adipose tissue (AD) overlying the posterior superior iliac crest were obtained from patients undergoing elective spine surgery. Chondrogenesis was induced using an established aggregate culture technique. Qualitative analysis was performed by histology and immunohistochemistry. DNA and glycosaminoglycan (GAG) quantitative assays were performed. Quantitative RT-PCR analysis was performed to compare expression of type II collagen between BM and AD aggregates. Osteogenic and adipogenic assays were also performed to confirm pluripotentiality of both AD-derived progenitor cells (ADPC) and BM-derived progenitor cells (BMPC). Toluidine blue metachromasia and type II collagen immunohistochemical staining were more extensive in the aggregates formed by BMPC. Quantitative RT-PCR showed a 500-5000 fold higher expression of type II collagen in the BMPC aggregates. The DNA content was 68% higher in the AD aggregates (p<0.02) but proteoglycan deposition per cell was 120% greater for BM-derived cell

Evidence level

Early human evidence such as case series or small samples is exploring possible benefits.

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