Cl- channels regulate lipid droplet formation via Rab8a expression during adipocyte differentiation.
Ouchi K., Yoshie S., Miyake M., Hazama A.
Animal Study, published in Biosci Biotechnol Biochem (2019) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Biosci Biotechnol Biochem (2019)
- Country
- England
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 31601151
- DOI
- 10.1080/09168451.2019.1677143
- Citations
- 1
Abstract (original English)
Several studies have shown that Cl - channels regulate the differentiation of some cell types. Thus, we investigated the role of Cl - channels on adipocyte differentiation using adipose tissue-derived stem cells (ASCs) and Cl - channel blocker. We induced rabbit ASCs into adipocytes using Cl - channel blocker. The expression levels of adipocyte markers were no significant difference between the cells treated with a Cl - channel blocker NPPB and untreated cells. However, when the cells were treated with NPPB, lipid droplets (LDs) sizes decreased compared with the untreated control. Interestingly, the expression levels of Rab8a, which is known as a regulator of LD fusion, were also decreased in the cells treated with NPPB. Other Cl - channel blockers, DIDS and IAA-94, also inhibited large LDs formation and Rab8a expression. These results demonstrate that Cl - channels do not regulate the adipocyte differentiation, but do regulate the LDs formation via Rab8a expression. Abbreviations : ASCs: adipose tissue-derived stem cells; LDs: lipid droplets; RUNX2: runt-related transcription factor 2; CFTR: cystic fibrosis transmembrane conductance regulator; TG: triacylglycerol; FA: fatty acid; GLUT4: glucose transporter type 4; ER: endoplasmic reticulum; ADRP: adipose differentiation-related protein; TIP47: tail-interacting protein of 47 kD; HSL: hormone sensitive lipase; PBS: phosphate-buf
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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