Co-culture with adipose mesenchymal stem cells promotes Blastocyst formation and gene expression in embryos from aged mice.
Hsuuw YD., Su YT., Chan WH., Wu CC., Tsai YC., Chen HC.
Laboratory Study, published in Regen Ther (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Regen Ther (2025)
- Country
- Netherlands
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 40242085
- PMCID
- PMC12002604
- DOI
- 10.1016/j.reth.2025.03.017
Abstract (original English)
Recent studies have highlighted the positive effects of co-culturing embryos with stem cells on embryo development in various mammalian systems. Stem cells secrete numerous factors, including cytokines, growth factors, and microRNAs, which promote embryo development. However, the impact of stem cells on the development of embryos derived from aged mice's oocytes remains poorly understood. This study evaluated the co-culture effects of adipose tissue-derived mesenchymal stem cells (ADMSCs) on zygotes, focusing on the developmental potential of fertilized embryos. Embryo quality was assessed through staining techniques to measure trophectoderm (TE), inner cell mass (ICM), and total blastocyst cell numbers during in vitro culture. Results demonstrated that ADMSC co-culture significantly improved zygote cleavage and blastocyst development rates, particularly in embryos derived from aged mice. Enhanced implantation and post-implantation potential were observed in embryos from both young and aged mice. Notably, co-culture increased TE, ICM, and total blastocyst cell numbers in aged mice-derived embryos without inducing apoptosis in blastocysts. Gene expression analysis revealed upregulation of OCT4 and G6PDH, associated with pluripotency and glucose metabolism, particularly in embryos from aged mice, while the heat stress marker HSP70 showed no significant changes. These findings dem
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
How we grade evidenceBrowse all related research
Filter the research library by this study's title keywords, author, or publication year.