Co-culture strategies for muscle-fat tissue development from caprine satellite cells: a step toward sustainable cultivated meat.
Elhaddad T., Thabet E., Essawy MM., Embaby AM., Hussein A., Elkhenany H.
Animal Study, published in Food Res Int (2025) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Animal Study
- Journal
- Food Res Int (2025)
- Country
- Canada
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 41185340
- DOI
- 10.1016/j.foodres.2025.117590
Abstract (original English)
Cultivated meat offers a sustainable alternative to conventional livestock production, yet caprine species remain underexplored. This study investigates the bipotential differentiation capacity of muscle satellite cells (MuSCs) isolated from Aleppo goats (Capra hircus), aiming to optimize dual myogenic-adipogenic tissue generation from a single cell source. MuSCs were isolated via mechanical and enzymatic digestion, displaying spindle-shaped morphology and positive expression of CD56, while negative for CD31. To investigate dual-lineage specification, five co-culture models were established, two simultaneous (M1: mixed co-culture, M2: layered co-culture) and three sequential (M3: adipogenic-to-myogenic, M4: same as M3 but long adipogenic priming, M5: myogenic-to-adipogenic). Simultaneous models involved combining or layering lineage-committed cells, while sequential models exposed a single MuSC population to staged adipogenic and myogenic cues. Gene expression analysis and histological staining revealed that M3 yielded optimal myogenic differentiation, characterized by high MyoD and MyoG expression. Model 5 favored adipogenesis, as evidenced by PPARγ expression and lipid accumulation alongside late myogenic traits. These findings present an efficient strategy for generating tailored muscle-fat constructs from caprine MuSCs and highlight their potential for cost-effective, susta
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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