SVF
Level A· Stronger Clinical EvidenceSystematic Review

Coculture of Chondrocytes and Stem Cells: A Review of Head and Neck Cell Lines for Cartilage Regeneration.

Lee MF., Steffens D., Chung JHY., Posniak S., Cheng K., Clark J.

Systematic Review with a reported sample of 10 on Osteoarthritis, Cartilage Damage, published in Cells Tissues Organs (2025) — summary generated from the PubMed abstract.

This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.

Study type
Systematic Review
Journal
Cells Tissues Organs (2025)
Country
Switzerland
Reported sample size
10
PMID
38513621
DOI
10.1159/000538461

Abstract (original English)

Bioprinting, using "bio-inks" consisting of living cells, supporting structures, and biological motifs to create customized constructs, is an emerging technique that aims to overcome the challenges of cartilaginous reconstruction of head and neck structures. Several living cell lines and culturing methods have been explored as bio-inks with varying efficacy. Coculture of primary chondrocytes and stem cells (SCs) is one technique well established for degenerative joint disease treatment, with potential for use in expanding chondrocyte populations for bio-inks. This study aimed to evaluate the techniques for coculture of primary chondrocytes and SCs for head and neck cartilage regeneration. A literature review was performed through OVID/Web of Science/MEDLINE/BIOSIS Previews/Embase. Studies reporting on chondrocytes and SCs in conjunction with coculture or cartilage regeneration were included. Studies not reporting on findings from chondrocytes/SCs of the head and neck were excluded. Extracted data included cell sources, coculture ratios, and histological, biochemical, and clinical outcomes. Fifteen studies met inclusion criteria. Auricular cartilage was the most common chondrocyte source (n = 10), then nasal septum (n = 5), articular (n = 1), and tracheal cartilage (n = 1). Bone marrow was the most common SC source (n = 9) then adipose tissue (n = 7). Techniques varied, with coc

What this study does not prove

  • • This study does not prove SVF is an approved treatment or a replacement for standard care.

Evidence level

Relatively higher-quality human studies compared with other topics in this database, e.g. multiple RCTs or systematic reviews. This does not mean it is standard or approved care.

How we grade evidence
ChondrocytesHumansCoculture TechniquesRegenerationStem CellsCell LineHeadCartilageNeckTissue Engineering
OsteoarthritisCartilage Damage
View on PubMedDOI

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