Collagenase from Lysinibacillus sphaericus: Production, Optimization and Application in Adipose-Derived Stem Cell Isolation.
Wang ZZ., Xue D., Qian JY., Wang K., Xu LF., Ma XD.
Laboratory Study, published in Appl Biochem Biotechnol (2026) — summary generated from the PubMed abstract.
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
- Level A · Stronger Clinical Evidence
- Level B · Emerging clinical evidence with positive signals
- Level C · Early human research exploring benefits
- Level D · Scientific groundwork from lab and animal studies
- Emerging · Emerging topic under active research
This page is generated from the PubMed record. The Thai description is an automated summary of bibliographic fields and the abstract, not a full translation, and is not medical advice.
- Study type
- Laboratory Study
- Journal
- Appl Biochem Biotechnol (2026)
- Country
- United States
- Reported sample size
- —
- Source database
- PubMed
- PMID
- 42287536
- DOI
- 10.1007/s12010-026-05761-3
Abstract (original English)
Collagenase, predominantly found in microorganisms, has significant potential for tissue separation and primary cell extraction. In this study, a collagenase Col51 derived from Lysinibacillus sphaericus JN-51 was isolated from sludge samples collected from aquaculture markets and slaughterhouses. Col51 was expressed in Bacillus subtilis (SCK6) and purified using a Ni-NTA column. Enzymatic characterization revealed that the enzyme exhibits optimal activity at 37 °C and pH 7.5. The presence of 10 mM Mg²⁺ and Ca 2 ⁺ enhances Col51 activity, whereas Zn²⁺ and Al³⁺ exert inhibitory effects. Through signal peptide optimization, Col51 expression increased from 53.5 U/mL to 164.8 U/mL in shake-flask experiments. Batch fermentation in a 7-L fermenter further enhanced enzyme activity, reaching a peak of 246.5 U/mL. In adipose stem cell isolation applications, Col51 demonstrated comparable separation efficiency to commercial collagenase I, with cell viability exceeding 90%, and cells exhibiting good morphology and stable growth characteristics. This study successfully identifies and optimizes Col51 collagenase from the non-pathogenic strain Lysinibacillus sphaericus, providing a novel tool enzyme for adipose stem cell isolation.
What this study does not prove
- • This study does not prove SVF is an approved treatment or a replacement for standard care.
- • This is preclinical work; animal or laboratory results cannot be applied to humans.
Evidence level
Evidence from laboratory and animal studies provides groundwork for understanding mechanisms and potential before human studies continue.
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